GST fusion wont stick

Pierce Chemical Co PierceChem at
Mon Nov 27 15:47:58 EST 1995

Many GST fusion proteins can be irreversibly bound to glutathione with the heterobifunctional 
crosslinker EDC.  This is a zero length crosslinker that links amine groups to carboxylic acid 
groups.  Both of these groups are readily available on the glutathione and at least some of them 
are probably availble on the GST protein.

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