carbohydrate help

Robert B. Hole rbh2 at Ra.MsState.Edu
Wed Nov 29 11:14:35 EST 1995

***forwarded for the author, please respond directly to him - and feel 
free to forward this to any appropriate venue!! thanks -RH***

I have two questions pertaining to the mucopolysaccharide jelly (very little
protein present) layers around frog eggs -->

        1. A suitable preservative has never been devised - any suggestions.
These layers sometimes stay when preserved in formalin but usually
disintegrate rather soon.  Need to maintain fairly normal shape, etc.
        2. The jellies are very transparent - any ideas on what to stain
with to add contrast?  Need to be able to see different membranes but remain
fairly transparent

tnx for the help, respond directly to ronn altig, rga2 at
R O N N  A L T I G - Drawer GY - Department of Biological Sciences -
Mississippi State University - Mississippi State, MS 39762 - TEL:
601-325-7561 (FAX: 7939) E-MAIL: rga2 at

forwarded by
Robert Hole
rbh2 at

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