HSA contamination in heparin - sepharose

Kurt L Brillhart klbrillhart at ccgate.dp.beckman.com
Wed Nov 29 15:20:48 EST 1995


In article <49fac9$oeo at adonis.clnsnet.ariadne-t.gr> Costas Apostolopoulos <capostol at atlas.uoa.gr> writes:
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>Subject: HSA contamination in heparin - sepharose
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>From: Costas Apostolopoulos <capostol at atlas.uoa.gr>
>Date: 28 Nov 1995 15:37:45 GMT
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>I am trying to purify a ribonuclease from human serum using a heparin - 
>sepharose chromatography as a first step. The problem is that I have a 
>significant contamination of HSA due to the bulk quantity of serum. Any help 
>would be appreciated.

>Thanx in advance....

You might want to try passing the serum over a cibacron blue-agarose column.  
The support is available from Sigma and is relatively specific for albumin.





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