membrane proteins on IEF gel?

Dima Klenchin klenchin at
Thu Nov 30 14:11:43 EST 1995

In article <49jstu$lin at>, Tuomo Glumoff <tglumoff at> wrote:
>I tried to purify an integral membrane protein on a flat-bed
>isoelectric focussing gel (preparative). I got bad results.

Could you be more specific on "bad results"? 
Also, do you care about denaturation? (8 M urea frequently helps).
What detergent? What apparatus? conditions? Ampholites used? etc?

- Dima

More information about the Proteins mailing list