membrane proteins on IEF gel?

Dima Klenchin klenchin at macc.wisc.edu
Thu Nov 30 14:11:43 EST 1995


In article <49jstu$lin at josie.abo.fi>, Tuomo Glumoff <tglumoff at btk.utu.fi> wrote:
>Hi,
>
>I tried to purify an integral membrane protein on a flat-bed
>isoelectric focussing gel (preparative). I got bad results.

Could you be more specific on "bad results"? 
Also, do you care about denaturation? (8 M urea frequently helps).
What detergent? What apparatus? conditions? Ampholites used? etc?

- Dima



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