Continuous fluorescamine assay

Malcolm White mfwhite at
Thu Nov 30 11:01:16 EST 1995

Maybe someone out there can help me.

I've just started to do protease assays, using an acetylated peptide
substrate and following the release of primary amine using fluorescamine
in a stopped assay.

Does anyone know whether the fluorescamine reaction can be followed in a
fluorimeter continuously, or whether there are any alternatives that fit
this requirement?

Thanks in advance,

Malcolm White

Malcolm White
University of Dundee

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