Reprobing PVDF membrane

sabah hussain mdsh at muscia.mcgill.ca
Thu Nov 30 10:44:14 EST 1995


Dear netters. I am doing alot of SDS-page electrophoresis and I am interested
in reporbing PVDF membrane more than once. Is there any protocol for stripping 
of primary-secondary antibody complex from PVDF membrane. I know that a such 
protocol exist for stripping nitrocellulose membrane (B. Parek, Analyt. Bioch. 1985). 
I appreciate any advice on this matter. 




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