membrane proteins on IEF gel?

Tuomo Glumoff tglumoff at btk.utu.fi
Thu Nov 30 04:18:54 EST 1995


Hi,

I tried to purify an integral membrane protein on a flat-bed
isoelectric focussing gel (preparative). I got bad results.
Does anyone have references/comments/experience to share to help
me decide, if I want to try again? Maybe the need for detergent
being present interferes with this method or something?

Tuomo Glumoff



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