Postdoctoral Position in Seattle
bassuk at u.washington.edu
Fri Sep 15 02:11:19 EST 1995
University of Washington Employment Opportunity
POSTDOCTORAL FELLOW / SENIOR FELLOW
Department: Biological Structure
Description of Research Project:
1. To generate mutations in the coding sequence of a recombinant protein
expressed in bacteria.
2. To isolate the recombinant, mutated protein by chromatography.
3. To compare the biological activities of the mutated protein to the
activities of the wild-type recombinant protein. Biophysical assays
will include circular dichroism and ultraviolet fluorescence emission
spectroscopies. Biological assays will include assays of endothelial
and glomerular mesangial cell behavior.
Background of Research Project:
The wild-type protein has been successfully expressed in bacteria as
a biologically active protein. The system has already yielded milligram
amounts of the protein from 1, 10, and 100 liter bacterial cultures. The
protein is believed to play a key role in the control of endothelial cell
behavior (with respect to angiogensis) and of mesangial cell behavior
(with respect to the resolution of glomerulonephritis).
This is an outstanding opportunity for a qualified individual
to join a multidisciplinary research group at a top research
medical center. Sufficient resources are available for the qualified
candidate to acquire state-of-the-art skills in protein chemistry. The
candidate will be encouraged to apply for extramural funding.
Requirements of Position:
Ph.D. in molecular/cellular biology or biochemistry. Hands-on research
experience in polymerase chain reaction, restriction enzymes, plasmid
manipulations, bacterial transformation, and DNA sequencing.
Computer literacy is essential.
Salary and length of support are negotiable.
To apply, send a resume and letter describing relevant experience
James Bassuk, PhD
Biological Structure, Box 357420
University of Washington
Seattle, WA 98195
More information about the Proteins