Blocking with Milk
hoekstra at unixg.ubc.ca
Thu Sep 21 20:20:55 EST 1995
Just a general question with regards to blocking membranes before
probing. I generally use a non-fat milk solution in TTBS.
Question: What are ideal dilutions to use? i.e. I've tried everything from
2%--> 10% Comments?
What is an ideal time period? Most "gaps" on the membrane (usually
nitrocellulose) should be blocked immediately upon addition of
blocking agent...it would be those so called "deep down pores/holes"
where a time period for blocking would be effective. I guess the
membrane pore size would also have an effect on the time period
required..right? Generally I use 1 hour at room temp with the 2% milk
More information about the Proteins