Blocking with Milk
ryang at SCRIPPS.EDU
Fri Sep 22 09:49:23 EST 1995
I usually use 10% milk in TTBS to block Immobilon-P PVDF membranes
(Millipore) at room temperature for 10min. The trick is you should
prepare milk solution immediately before use.
The Scripps Res.Inst.
On 22 Sep 1995, Sucking Gippy wrote:
> Just a general question with regards to blocking membranes before
> probing. I generally use a non-fat milk solution in TTBS.
> Question: What are ideal dilutions to use? i.e. I've tried everything from
> 2%--> 10% Comments?
> What is an ideal time period? Most "gaps" on the membrane (usually
> nitrocellulose) should be blocked immediately upon addition of
> blocking agent...it would be those so called "deep down pores/holes"
> where a time period for blocking would be effective. I guess the
> membrane pore size would also have an effect on the time period
> required..right? Generally I use 1 hour at room temp with the 2% milk
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