help, protein dies upon dilution

Venkatachalam K. V. venk at popserver.nih.gov
Mon Sep 25 09:47:15 EST 1995


In article <438dvf$1858 at otho.cc.flinders.edu.au>,
Gordon.Lehmann at flinders.edu.au (Gordon) wrote:

> In message <NATHAN.95Aug31183535 at protos.bms.com> - nathan at protos.bms.com (Natha
> n O. Siemers) writes:
> :>
> :>
> :>Are you sure that the protein is not sticking to the surface of your
> :>container when you dilute it?  Can you "block" a tube by first filling
> :>with a bsa soln, removing it, then adding your protein and diluting?
> :>If so, alternative materials could be investigated, such as silanized
> :>glass.  Perhaps others on the net have other suggestions for materials...
> :>
> :>nathan
> :>
> :>>>> On 31 Aug 1995 15:10:40 -0700, tan1 at NIEHS.NIH.GOV ("Tan.Zheng") said:
> :>
> :>Tan> Hi, Dear colleague: I am working on a protein that losses
> :>Tan> activity when it is diluted. This can be prevented by adding BSA
> :>Tan> to the protein. But I need the protein as pure as possible and
> :>Tan> dilution can not be avoided. I'll appreciate very much if any one
> :>Tan> can share with me any ideas of stabilizing such a protein without
> :>Tan> adding exogenous proteins.  Thanks in advance.  Z.Tan
> :>Tan> tan1 at niehs.nih.gov
> :>
> :>-- 
> :>Nathan O. Siemers, Ph.D.	
> :>Bristol-Myers Squibb Pharmaceutical Research Institute
> :>3005 First Avenue, Seattle, WA 98121
> :>(206) 727-3741  	siemers at bms.com
> :>"You want fries with that?"
> 
> 
> 
> Try Polyethyleneglycol (PEG) upto 20%
> 
> ----------------------------------------------------------------------
> Gordon.Lehmann at flinders.edu.au
> ----------------------------------------------------------------------

Try final conc of 10% v/v  glycerol or Sucrose



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