protein concentration

James Fethiere James.Fethiere at BRI.NRC.CA
Wed Sep 27 00:40:32 EST 1995


What would be the correct way of getting protein concentration from the
extinction coefficient.  I have been told to just read the absorbance at
280, sum up the individual extinction coefficients of Tyr, Trp, and Cys,
and use the formula to get my concentration.  This seems pretty reasonable
to me, but i was wondering how the accuracy of this method compares to the
standard protein assay. 



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