transient expression - help

laura d. kramer ldk at mendel.berkeley.edu
Tue Sep 26 20:20:18 EST 1995


I would like to transfect yeast cells with synthetic mRNAs generated from
T7-mediated transcription of gus:viral gene fusions cloned into
pBluescript.  The aim is to determine the site of localization of such
fusions.  Any advice on the design of such experiments would be greatful. 
Are such transfections most easily (efficiently) accomplished using
lipofection or electroporation?  Please reply by email.

Thank you,

Michael Goodin
mgoodin at nature.berkeley.edu



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