Blocking with Milk

Flip hoedemaeker fhoedem at oci.utoronto.ca
Wed Sep 27 08:37:20 EST 1995


If it's any help, try adding the first antibody directly in the blocking
solution (after 1 hour of blocking of course). It always works for me.
BTW, I use PVDF membranes, not nitrocellulose. I find background is much 
lower on Western blots... Flip




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