Elution Conditions from Affinity Resin??

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Thu Sep 28 03:19:39 EST 1995


Stephen Gately (sg at nwu.edu) wrote:
> I would be interested in any protocol for eluting from an affinity column
> other than increasing NaCl concentrations.  Unfortunately the enzyme I am
> eluting is inactivated by increasing salt concentrations.

Dependent on your resin you could try to elute with the bound ligand.
(Of course not feasible for resins where the ligand is a protein.)

--Cornelius.

--
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany        email: phak004 at rzbox.uni-wuerzburg.de */
/* "Science is the game we play with God to find out what His rules are."  */



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