Refolding a GST-fusion-protein --- Help needed!
nzheng at el
Fri Sep 29 15:56:12 EST 1995
I have never worked on GST-fusion-proteins but you might be interested in
the following article:
"Solubilization and Purification of Enzymatically Active Glutathione
S-Transferase (pGEX) Fusion Proteins"
John V. Frangioni and Benjamin G. Neel.
Analystical Biochemistry 210, 179-187 (1993)
Basically, the authors have developed a protocol to purify insoluble GST
fusion proteins using sarkosyl. I read this when I had problem with my
His-tagged protein in inclusion bodies.
Wulf Blankenfeldt (wub at gbf-braunschweig.de) wrote:
: Dear all,
: we are currently working on a GST-fusion-protein using the pGEX-2T
More information about the Proteins