Driving Carboxypeptidases backwards
lsc at novo.dk
Fri Jan 5 19:56:19 EST 1996
Jennifer L. Potter wrote:
> I am thinking about adding a C-terminal tyrosine onto my protein of
> interest. To bypass all of the molecular biology, it was suggested
> that I simply drive a carboxypeptidase (probably either A or Y) reaction
> backwards to ADD a tyr instead of cleave the C-terminal residue (a Ser).
Enzymatic coupling has been used extensively for trypsin.
In this case you drive the reaction by running it in about 70% organic
solvent (e.g. DMF/DMSO/1,4-Butanediol) which shifts the equilibrium
I don't know whether CpA or CpY works the same way (how about a little
trip to the library?)
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