fresh transformations for protein expression??

Manoj Ramjee mkr at
Fri Jan 5 03:41:22 EST 1996

I used to work in a lab which encountered the same problem, but with 
yeast (S. cerevisiae).  They put it down to recombination events 
that affected the expressed gene.  I'm not sure about BL21 and its 
recombination ability, but could this be a possibility ?


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