His-tagged proteins

Vadim Tsvetnitsky vadtsvet at biochem.ou.dk
Thu Jan 11 05:27:30 EST 1996


Hi,

Has anyone tried to regenerate Ni-NTA agarose used for purification of his-tagged proteins?
I understand from the broushure that a particular batch can only be used 3 to 5 times for the
purification of one protein and should be trashed. Is it so?

Thanks.

Vadim Tsvetnitsky
vadtsvet at biochem.ou.dk



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