Chemiluminescence

Tori Brophy torih at genetics.washington.edu
Mon Jan 15 15:37:28 EST 1996


jbooth at ACS.UCALGARY.CA (Joseph Boothe) wrote:
>I haven't tied others, but the Amersham system has worked well for me 
>using both nitrocellulose and PVDF membranes. I have seen approximately a 
>10-fold increase in sensitivity over the alkaline phosphatase NBT/BCIP 
>system. Hope this helps.
>
>On 15 Jan 1996, Michael Daws wrote:
>
>> A quick question - what is the best chemiluminescent agent available for 
>> western blotting, and which membrane works best with it?
>> 

I use the Pierce HRP system with PVDF membranes.  It works well for me and costs 
half as much as the Amersham HRP system.  

Tori Brophy
 





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