torih at genetics.washington.edu
Mon Jan 15 15:37:28 EST 1996
jbooth at ACS.UCALGARY.CA (Joseph Boothe) wrote:
>I haven't tied others, but the Amersham system has worked well for me
>using both nitrocellulose and PVDF membranes. I have seen approximately a
>10-fold increase in sensitivity over the alkaline phosphatase NBT/BCIP
>system. Hope this helps.
>On 15 Jan 1996, Michael Daws wrote:
>> A quick question - what is the best chemiluminescent agent available for
>> western blotting, and which membrane works best with it?
I use the Pierce HRP system with PVDF membranes. It works well for me and costs
half as much as the Amersham HRP system.
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