His-tagged proteins

[Rob]

Hi,
 I use this resin routinely to purify histagged Photoactive Yellow 
Protein on large scale (100 mg -scale). After use I wash the column with 
5 volumes elution buffer,  5 volumes 0.2 mM aceticacid, 5 volumes bidest 
and then with one volume of ZnSo4 to recharche the resin.After washing 
excess metal from the column with bidest I store the material in 20 % 
ethanol.
I have used one batch over 5 times (for the same protein of course) and 
still had only a minor loss in protein binding capacity.
I hope that this serves you,
Greetings,  Arthur.