Extracting small amounts of protein from SDS-PAGE
mkr at mole.bio.cam.ac.uk
Fri Jan 19 10:27:35 EST 1996
Hi there folks,
I have a friend who has a very small amount (just visible by
Coomasie and clearly visible by silver stain) of protein on an
SDS-PAGE gel. She would like to get it out of the gel. We
suggested cutting out the band followed by electro-elution. Others
suggested dialysis, and yet others suggested transferring onto
nitrocellulose and then dissolving the nitrocellulose.
Does anyone have a tried and tested method for getting small amounts
of protein out of a PAGE gel ?
Thank you for your help.
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