fresh transformations for protein expression??

ohannes1 at jeflin.tju.edu ohannes1 at jeflin.tju.edu
Mon Jan 22 17:29:11 EST 1996


Hello,

Re: DMSO vs. glyOH 

 I have used 7% DMSO 	for several years added directly  to a freshly saturated 
culture and have found  it every bit as good if not better than glycerol for freezer stocks.
	Its certainly easier to pipet.  		Cheers!

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From: krasel at wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel)
Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins
Followup-To: bionet.molbio.methds-reagnts,bionet.molbio.proteins
Subject: Re: fresh transformations for protein expression??
Date: 16 Jan 1996 12:12:28 GMT
Organization: University of Wuerzburg, Germany
Message-ID: <4dg4nc$pnc at winx03.informatik.uni-wuerzburg.de>
References: <4ch87s$15oc at majestix.uni-muenster.de> <msz-1101961331140001 at image.bmc.uu.se> <DL0wEo.L78 at uns.bris.ac.uk> <30FAE763.4A4A at novagen.com>

Jennifer Lyon (JLyon at novagen.com) wrote:
> How much glycerol are you using when you make a glycerol stock. We've 
> found that 8% is sufficient, and if you use too much glycerol it makes 
> plasmids extremely unstable.

Anybody who has experience with DMSO instead of glycerol? (I use it,
but I haven't rigorously compared it with glycerol.)

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP3 */
/* "Science is the game we play with God to find out what His rules are."  */


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