soluble protein expression in E. coli

ohannes1 at ohannes1 at
Mon Jan 22 17:12:05 EST 1996

Dear Saverio,

	One way  express some proteins so that they are soluble is to express them from a vector  that 
puts a leader sequence on them so that they are secreted into the periplasmic space  between the 
plasma membrane and the bacterial cell wall.  This is a more reducing environment and avoids some 
folding related problems that can result in insolubility.   Several vectors have these sequences 
including pINIIIOmpA2 (M. Inouye) also NEB's pMal-P2 as well as others.

																																				                                                        Good Luck,

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