gelatin agarose & HSP70

Michael Vork Michael.Vork at FYS.rulimburg.nl
Tue Jan 23 05:44:02 EST 1996


In article <1996Jan19.150208.71705 at cc.usu.edu> sl16s at cc.usu.edu (NAME RAVI PRAKASH .D) writes:
>Path: rl0001.rulimburg.nl!news.nic.surfnet.nl!howland.reston.ans.net!swrinde!elroy.jpl.nasa.gov!usc!sdd.hp.com!hamblin.math.byu.edu!park.uvsc.edu!news.cc.utah.edu!news.cs.utah.edu!cc.usu.edu!sl16s
>From: sl16s at cc.usu.edu (NAME RAVI PRAKASH .D)
>Newsgroups: bionet.molbio.proteins
>Subject: gelatin agarose & HSP70
>Message-ID: <1996Jan19.150208.71705 at cc.usu.edu>
>Date: 19 Jan 96 15:02:07 MDT
>Organization: Utah State University
>Lines: 6

>I am trying to purify HSP70 using gelatin affinity chromatography acc to Nandan
>et al J of Imm Meth 176(1994) 255-263. Does any one have any experience with
>this sort of purification?? Any suggestions or tips would be helpfull. Thanks
>in advance.


Don't try it. I have tried to purify HSP70 with every possible method. Did 
not work due to the sticky nature of the protein. I advise you to produce it 
in E. Coli. Sequnces are readily available.

Good luck,

Michael



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