gelatin agarose & HSP70

Michael Vork Michael.Vork at
Tue Jan 23 05:44:02 EST 1996

In article <1996Jan19.150208.71705 at> sl16s at (NAME RAVI PRAKASH .D) writes:
>From: sl16s at (NAME RAVI PRAKASH .D)
>Newsgroups: bionet.molbio.proteins
>Subject: gelatin agarose & HSP70
>Message-ID: <1996Jan19.150208.71705 at>
>Date: 19 Jan 96 15:02:07 MDT
>Organization: Utah State University
>Lines: 6

>I am trying to purify HSP70 using gelatin affinity chromatography acc to Nandan
>et al J of Imm Meth 176(1994) 255-263. Does any one have any experience with
>this sort of purification?? Any suggestions or tips would be helpfull. Thanks
>in advance.

Don't try it. I have tried to purify HSP70 with every possible method. Did 
not work due to the sticky nature of the protein. I advise you to produce it 
in E. Coli. Sequnces are readily available.

Good luck,


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