glycosylated protein on SDS gel

Warren Gallin wgallin at gpu.srv.ualberta.ca
Wed Jan 24 12:06:11 EST 1996



In Article <Pine.ULT.3.91.951221165356.17530A-100000 at banyan>,
stones at GROVE.UFL.EDU (Ke Wu) wrote:
>Hi, there :
>
>Anybody knows whether SDS will mask the increase of protein mass caused by
>glycosylation on a SDS gel? Thank in advance.


Usuakky, no.  Typically you can tell if a protein is N-glycosylated by
comparing the mobility of the protein with and without digestion of
endoglycosidase F.  In fact, you can often tell how many glycosylation sites
there are by running a set of serial 2-fold dilutions of the enzymes and
counting the intermediate forms.

Warren Gallin,
Department of Biological Sciences, University of Alberta
wgallin at gpu.srv.ualberta.ca



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