Glycerol concentration

Keld Sorensen KeldS at
Mon Jan 29 11:29:45 EST 1996

michael.didonato at (Michael DiDonato) wrote:
>I would like to know what concentration of glycerol is used to help keep
>proteins soluable and stable when frozen.
>University of Toronto

You may want to also take a look at 
ethylene glycol which offers the same protection,
but remains at a very low viscosity.
Both are used at 25-50% 

Make sure you get really CLEAN stuff - both
products are most often sold in a commercial/technical
grade which may or may not kill your enzyme/protein.

Some vendors have purified versions.


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