removing nucleic acids from extracts
Dr. Jeffrey F.D. Dean
jdeanx1 at uga.cc.uga.edu
Tue Jan 30 08:17:19 EST 1996
trey simmons wrote:
> Hello all; I'm hoping someone can help with what I think should be a
> straightforward question. I'm purifying a protein in E. coli using
> Any suggestions would be appreciated.
> Trey Simmons
> Gladstone Institute for Cardiovascular Disease
> San Francisco, Ca.
One of the first lessons I was taught as a new graduate student was the
effectiveness of protamine sulfate for precipitating nucleic acids from
bacterial lysates. I have infrequently come across proteins that
co-precipitate, but I still use this method with bacterial, plant,
and fungal extracts. Try slow addition of 1:20 vol of a 2% solution of
protamine sulfate in extraction buffer. Stir at 4°C and a precipitate
should form straight away.
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