LECTIN IMMUNOBLOTTING...HELP

Flip Hoedemaeker fhoedem at oci.utoronto.ca
Tue Jan 30 13:17:04 EST 1996


Surgical Residence wrote:
> 
> I'm trying to do a lectin immunoblot with BM's ConA-DIG.
> Can I do this with proteins run on a denaturing system like SDS, or is
> it only possible with a non-denaturing (CHAPS) ststem?
> 
> Any other tips are also appreciated.

Lectins bind to the sugar moiety of your protein, which is unaffected by 
SDS.....

Flip



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