Restriction proteases?

Leemor Joshua-Tor leemor at CSHL.ORG
Tue Jan 30 10:22:20 EST 1996


>In article <v01530506ad315f0bd728@[143.48.60.60]>,
>   leemor at CSHL.ORG (Leemor Joshua-Tor) wrote:
>..
>>There is a very nice protease, which is very specific, and so very
>>useful for what you want to do called TEV protease. Highly recommended.
>>It will not cut in any known protein and is the purest and
>>the best buy of units activity. ...
>
>What's the specificity?
>
>
>Jonathan B. Marder                 ,      Department of Agricultural Botany
>E-mail: MARDER at agri.huji.ac.il     |      The Hebrew University of Jerusalem
>Phone: (08 or +9728) 9481918       | /\/  Faculty of Agriculture
>Fax:   (08 or +9728) 9467763       |/  \  P.O.Box 12, Rehovot 76100, ISRAEL
>URL: http://indycc1.agri.huji.ac.il/~marder


The specificity of TEV protease:

Glu-Asn-Leu-Tyr-Phe-Gln-I-Gly.  The Gly is left on fusion protein.


*******************************************************************
  Leemor Joshua-Tor
  Cold Spring Harbor Laboratory           Tel. (516) 367 8821
  1 Bungtown Road                         Fax  (516) 367 8873
  Cold Spring Harbor, NY 11724            e-mail: leemor at cshl.org
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