glycosylated protein on SDS gel

dslomczy at mailbox.syr.edu dslomczy at mailbox.syr.edu
Wed Jan 31 06:47:12 EST 1996


Yes glycosylated proteins will act strangely.  There was two papers out in
Biochemistry by Leach, Colwan, and Fish about measuring molecular mass by
SDS-electrophoresis and other methods.  There conclusions were the glycoproteins bind
enough SDS (the glycosylation doesnot bind SDS), so the charge to mass ratio is off.  One
can expect between -50 to +50% change with glycosylated proteins.  I hope this helps.

                       Sincerely,
                         David Slomczynski
                         SUNY-ESF
                         444 Illick Hall
                         Syracuse, NY  13090-3252




More information about the Proteins mailing list