phage promoter + AUG vectors for IVTT?

cok at mole.bio.cam.ac.uk c.okane at gen.cam.ac.uk
Wed Jul 3 08:22:46 EST 1996


We'd like to express 35S-labelled protein domains in vitro. They don't
have their own ATG initiation codon. Can anyone recommend any vector
series with a standard phage promoter (T3, T7, SP6) and an ATG which is
known to work efficiently in a system like Promega TNT, followed by a
multiple cloning site - preferably there should be three vectors with the
polylinker staggered to allow use of all three reading frames with the
same restriction sites.

Thanks,
Cahir
c.okane at gen.cam.ac.uk

-- 
Cahir O'Kane, Dept of Genetics, Univ. of Cambridge, UK
cok at mole.bio.cam.ac.uk



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