E.coli proteins retained on Ni-column

Scott McMahan mcmahan at oncology.wisc.edu
Fri Jul 5 11:53:21 EST 1996


In article <federici-0407961606580001 at federici.cochin.inserm.fr>,
federici at icgm.cochin.inserm.fr (Federici) wrote:


> But, due to their amino acid content, some E.coli proteins are also bound
> to the Ni-column, and disturb the purification of the recombinant protein.
> Has anybody identified E.coli proteins which are retained during
> purification using a Ni-column?
> I would also be very interested in references describing this subset of
> E.coli proteins. 


Check out Wulfing et al Journal Biol Chem 269(4) 2895-2901.  They discuss
a protein (WHP) which does bind to a Ni-column.

-- 
                                         Scott McMahan
                                         mcmahan at oncology.wisc.edu



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