Affinity Chromatography

Bob Hoesch Bob_Hoesch at fws.gov
Mon Jul 8 15:10:26 EST 1996


Can someone suggest a reference for affinity chromatography of whole antiserum 
to adsorb unwanted specificities?  For example, I have rabbit antiserum 
against whole deer serum.  I want to adsorb out the components which cross 
react with bovine proteins.  I was thinking of using epoxy-activated Sepharose 
to bind with whole bovine serum, reacting with the anti-deer on a column, 
washing with PBS and eluting with high pH buffer.

Any references or comments would be very welcome.

Thanks.

Bob Hoesch
U.S. Fish & Wildlife Forensic Laboratory
Ashland, OR



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