Not binding in Western

Charles Brenner brenner at dada.jci.tju.edu
Tue Jul 9 11:17:46 EST 1996


Gourley, Tania Suzanne wrote:
> 
> Does anyone know of any references refering to antibodies which cannot
> recognise a protein when it is bound to membrane such as in Western blots but
> can bind free protein.
> 
> Thanks,
> Tania
If an antibody is raised to native protein it wouldn't be at all 
surprising if epitopes are native.  Conversely, antibodies raised 
against SDS-PAGE-run samples may work fine on Western and fail in IP.

Charlie 
-- 
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Charles Brenner                         Laboratory of Protein Structure
Assistant Professor                               and Cellular Function
Kimmel Cancer Institute                            phone (215) 503-4573
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