gel electrophoresis of peptides...

Thomas Wilkinson wilkinst at deft.cc.purdue.edu
Wed Jul 10 17:11:23 EST 1996


Newsgroups: bionet.molecules.peptides
Subject: gel electrophoresis of peptides...
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Distribution: world
Organization: Purdue University Computing Center
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Hello,

I was wondering if somebody had some experience with SDS gel electrophoresis of
peptides.  I have a sample that I would like to analyze to see if it contains
a short (12-residue) peptide, molecular weight = 1462.

The sample is not some mixture of many species...the sample to be analyzed
either has the 12-mer, or else the sample contains no peptide whatsoever.  Thus,
this gel should be really easy to interpret.  I also have a similar sample that
I would like to analyze for peptide content, except in this case I am looking
for the presence or absence of a 33-residue peptide (MW = 3487).

I would like to run these samples out on an SDS gel, and then coomassie-stain.  
My question is that I don't know what the optimal percentage of polyacrylamide 
would be for this task.  What percent gel is best?  Is gel electrophoresis of
peptides commonly done?  Your suggestions are much appreciated.

thanks,

tom




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