gel electrophoresis of peptides...
wilkinst at deft.cc.purdue.edu
Wed Jul 10 17:11:23 EST 1996
Subject: gel electrophoresis of peptides...
Organization: Purdue University Computing Center
I was wondering if somebody had some experience with SDS gel electrophoresis of
peptides. I have a sample that I would like to analyze to see if it contains
a short (12-residue) peptide, molecular weight = 1462.
The sample is not some mixture of many species...the sample to be analyzed
either has the 12-mer, or else the sample contains no peptide whatsoever. Thus,
this gel should be really easy to interpret. I also have a similar sample that
I would like to analyze for peptide content, except in this case I am looking
for the presence or absence of a 33-residue peptide (MW = 3487).
I would like to run these samples out on an SDS gel, and then coomassie-stain.
My question is that I don't know what the optimal percentage of polyacrylamide
would be for this task. What percent gel is best? Is gel electrophoresis of
peptides commonly done? Your suggestions are much appreciated.
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