gel electrophoresis of peptides...

Lauge Schaffer lsc at novo.dk
Fri Jul 12 06:09:55 EST 1996


Thomas Wilkinson wrote:
> 
> Newsgroups: bionet.molecules.peptides
> Subject: gel electrophoresis of peptides...
> Summary:
> Followup-To:
> Distribution: world
> Organization: Purdue University Computing Center
> Keywords:
> 
> Hello,
> 
> I was wondering if somebody had some experience with SDS gel electrophoresis of
> peptides.  I have a sample that I would like to analyze to see if it contains
> a short (12-residue) peptide, molecular weight = 1462.
> 
> The sample is not some mixture of many species...the sample to be analyzed
> either has the 12-mer, or else the sample contains no peptide whatsoever.  Thus,
> this gel should be really easy to interpret.  I also have a similar sample that
> I would like to analyze for peptide content, except in this case I am looking
> for the presence or absence of a 33-residue peptide (MW = 3487).
> 
> I would like to run these samples out on an SDS gel, and then coomassie-stain.
> My question is that I don't know what the optimal percentage of polyacrylamide
> would be for this task.  What percent gel is best?  Is gel electrophoresis of
> peptides commonly done?  Your suggestions are much appreciated.
> 
> thanks,
> 
> tom

This sounds like sending your lunch-box off for elemental analysis to
figure out whether you have eaten your sandwiches or not.
In your case it sounds like an amino acid analysis, a few steps of
peptide sequencing, a mass-spec or a protein determination (e.g.
Bradford or Lowry) would give you better information.

Lauge



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