gel electrophoresis of peptides...

Stephen P. Driska PhD driska at astro.ocis.temple.edu
Fri Jul 12 13:54:42 EST 1996


Thomas Wilkinson (wilkinst at deft.cc.purdue.edu) wrote:
: Newsgroups: bionet.molecules.peptides
: Subject: gel electrophoresis of peptides...
: Summary: 
: Followup-To: 
: Distribution: world
: Organization: Purdue University Computing Center
: Keywords: 


: Hello,

: I was wondering if somebody had some experience with SDS gel electrophoresis of
: peptides.  I have a sample that I would like to analyze to see if it contains
: a short (12-residue) peptide, molecular weight = 1462.

: The sample is not some mixture of many species...the sample to be analyzed
: either has the 12-mer, or else the sample contains no peptide whatsoever.  Thus,
: this gel should be really easy to interpret.  I also have a similar sample that
: I would like to analyze for peptide content, except in this case I am looking
: for the presence or absence of a 33-residue peptide (MW = 3487).

: I would like to run these samples out on an SDS gel, and then coomassie-stain.  
: My question is that I don't know what the optimal percentage of polyacrylamide 
: would be for this task.  What percent gel is best?  Is gel electrophoresis of
: peptides commonly done?  Your suggestions are much appreciated.

: thanks,

: tom
	No experience with anything like that, but I would recommend Novex
 (800)456-6839, a company that sells equipment and supplies including 
precast gels
using the tris-tricine gel system that claims to be able to resolve
 peptides about as small as those you mention.  Good luck


--
Steve Driska, Physiology Department, Temple University Medical School
Philadelphia, PA 19140 USA (215) 707-3283 
driska at astro.ocis.temple.edu  
If I could think of something witty and clever to say, it would go right 
here ----->>    .........................



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