elution of an his-tag protein

Falson falson at verlaine.saclay.cea.fr
Fri Jul 19 11:26:49 EST 1996


dear netters,
I try to purify a solubilized his-tagged membrane protein. Binding of 
the protein on NTA is OK but it seems that only 10% of the protein can 
be eluted by a gradient of imidazole ranging from 30 mM to 0.5 M.
I would like to know if other persons had the same problem.
thank you



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