solubilisation for 2-D gels

Laurence Barker l.barker at AUCKLAND.AC.NZ
Mon Jul 22 19:36:23 EST 1996


Hello, 
I am attempting to run membrane proteins on a 2-D gel, but am 
experiencing no separation in the IEF first dimension. I have tried using 
0.5% Triton X-100 in the solubilisation buffer, and have now been 
informed that minimal SDS/DTT might help also. Does anyone know about the 
use of chloryl hydrate to help in solubilisation/inhibition of 
aggregation, if that is what is happening ?
Any help glady accepted,

Laurence Barker.

The Horticultural and Food Research Institute of New Zealand, 
Mt Albert Research Centre,
Auckland, 
New Zealand
l.barker at auckland.ac.nz





More information about the Proteins mailing list