achim at ibex.ca
Mon Jul 22 15:16:57 EST 1996
Ayari Sahbi wrote:
> I know that a dimeric protein will separate during SDS-PAGE, but is this
> dimer will separate in his two subunits when it is in his native form,
> during non-dissociating PAGE?
> Kind regards,
> Sahbi Ayari.
Not necessarily. If the subunits are crosslinked by SS-bridges, they will definitely
stay together under non-reducing conditions. But I know of at least one example,
L-leucin dehydrogenase from Bacillus subtilis, where the electric field during native
PAGE is not strong enough to disrupt the bond between the subunits caused by hydrophobic
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