protein electrophoresis

[Achim Recktenwald]

Ayari Sahbi wrote:
> 
> Hi
> 
> I know that a dimeric protein will separate during SDS-PAGE, but is this
> dimer will separate in his two subunits when it is in his native form,
> during non-dissociating PAGE?
> 
>  Kind regards,
>  Sahbi Ayari.


Not necessarily. If the subunits are crosslinked by SS-bridges, they will definitely 
stay together under non-reducing conditions. But I know of at least one example, 
L-leucin dehydrogenase from Bacillus subtilis, where the electric field during native 
PAGE is not strong enough to disrupt the bond between the subunits caused by hydrophobic 
or salt-bridges.


Achim