elution of an his-tag protein
KeldS at uic.edu
Tue Jul 23 18:15:21 EST 1996
Well, by "stripping" the Ni off the column the protein
will come down as well - a quickie dialysis and you
get rid of the Ni - then you reload the column by putting
Ni on it.
This elution will tell you whether the protein is stuck
on the Ni or on the support.
It also has the advantage that you see the elution as a
bluish band (the green nickel turns blue with EDTA).
just my 2 cents worth on the subject.
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