HELP: electroblot a Coomasie Stained PAG onto a Membrane

campa002 at mc.duke.edu campa002 at mc.duke.edu
Wed Jul 24 12:02:36 EST 1996


Dear Vic,
	What usually happens is that the stain transfers very well but the 
proteins remain in the gel. A more successful approach may be to 
transfer the proteins from an unstained/unfixed gel onto the membrane, 
then stain the membrane with a reversible stain. Or run two gels, 
stain one and transfer the other. You can then stain the gel after 
transfer to see what didn't move. Sometimes it helps to sling the gel 
against the wall if nothing else works.

Mike



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