HELP: electroblot a Coomasie Stained PAG onto a Membrane

[campa002 at mc.duke.edu]

Dear Vic,
	What usually happens is that the stain transfers very well but the 
proteins remain in the gel. A more successful approach may be to 
transfer the proteins from an unstained/unfixed gel onto the membrane, 
then stain the membrane with a reversible stain. Or run two gels, 
stain one and transfer the other. You can then stain the gel after 
transfer to see what didn't move. Sometimes it helps to sling the gel 
against the wall if nothing else works.

Mike