HELP: electroblot a Coomasie Stained PAG onto a Membrane

Ke Wei victorke at acpub.duke.edu
Wed Jul 24 15:13:01 EST 1996


Hi, Guys:

I attempted to electroblot a piece of Coomassie blue stained (fixed in 
Acetic Acid/MetOH) polyacrylamide gel onto a PVD membrane.  The 
efficiency was too low.  I'm afraid that I could not transfer fixed 
proteins onto any membrane.  Does anyone have a good idea as how to remedy 
the problem?  Any nice protocol in electrobloting Coomassie blue 
stained/fixed proteins will be very much appreciated!  Thanks for your 
attention.


Vic



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