Raising antibodies to BSA conjugate

Satish Nair satish at a.chem.upenn.edu
Fri Jul 26 00:26:00 EST 1996



Hello,

	I intend to raise polyclonals against a small molecule that I
can conjugate to BSA. The small molecule forms a Schiff base with lysine
residues of BSA. Will the Schiff base be stable for raising antibodies.
?
I could presumably stabilize the linkage by reduction with borohydride,
but may end up with toxic boron end-products. Does anyonoe have experience
with this situation?

Thanks,
Jonathan Goldberg.
  email- goldbej at jkdec.rockefeller.edu




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