proteins chromatography

Bilbrey Robert C478SCB at SEMOVM.SEMO.EDU
Fri Jul 26 09:55:43 EST 1996


On 24 July, Sbouhal sent the following post:

>I would like to separate a protein (Mr = 20000) from its modified form (blocked
>lysine residue) by ion exchange chromatography. Anyone has a suggestion
>on chromatographic conditions ?
>
I would suggest hydroxyapatite for a separating matrix if the number of
exposed free amine groups is different. The best review of this
technique that I have found is in Deutscher, Guide to Protein
Purification, Methods in Enzymology V 182.

Robert Bilbrey
c478scb at semovm.semo.edu
Biology Dept
Southeast Missouri State Univ




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