HOW WIDE IS ANTIBODY'S BITE?

Michael J. Tuvim mtuvin at bcm.tmc.edu
Tue Jul 30 01:02:23 EST 1996


HOW WIDE IS ANTIBODY'S BITE?

Dear folks!

Could anyone tell me what is the necessary length of a spacer 
between two repeated epitopes? 

I am trying to design an expression construct tagged with a couple of 
Hemagglutinin epitopes (YPYDVPDYA). I'd like to have two of them in a 
row in order to get a stronger signal in transfected cells. Now, how long 
should be a spacer in between two of them? I don't want two primary 
antibodies bumping into each other and not being able to sit peacefully 
side by side.

In other words: how much space an immunoglobulin requires besides the 
epitope itself?

Your help is greatly appreciated.

Please kindly consider emailing your response to me in addition to your 
posting. My address is mtuvin at bcm.tmc.edu

Thank you 
Michael Tuvim



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