inducible eukaryotic expression

Mike atkinson at gsf.de
Tue Mar 5 06:20:41 EST 1996


In article <vertino_bell-2902961614120001 at cbmf36.cber.fda.gov> Annette,
vertino_bell at cber.cber.fda.gov writes:

...Problems with inducible expression.

Like many I was seduced by the Stratagene protocol on lac induction. We
made the lines and found that the presence of a reporter AND a test
plasmid in our repressor lines causes leaky repression.I guess the
problem is that we dont get enough lac repressor expression. I still cant
get Stratagene to send me a list of papers citing sucessful use of the
system.



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