Nickel agarose for His tag

[Daidalos]

In article <4h05hf$seg at larry.rice.edu>, "Eugenio L. de Hostos"
<hostos at bioc.rice.edu> wrote:

> Hi,
> anybody out there know how to make Ni2+-agarose for affinity
> chromatography of His-tagged proteins?
> 
> Thanks,
> Eugenio L. de Hostos, Ph.D.
> Department of Biochemistry and Cell Biology, MS 140
> Rice University
> email hostos at bioc.rice.edu
> URL http://bioc.rice.edu/~hostos

Pharmacia make a chelating sepharose support, haven't heard of
Chelating agarose for his-tagging though.

Orhan.

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