I have a question about equilibrium dialysis to find a binding constant
for a product to an enzyme. I only plan this to be a one time thing and
was wondering if there was a reference out there that explains the set up
and maybe how to do this simply with things in the lab instead of buying
an equilibrium dialysis setup.
I expect the Kd to be in the submicromolar range and the enzyme is soluble.
Any help appreciated. Thanks in advance.