Equilibrium dialysis

[William P. Tschantz]

Hi 


I have a question about equilibrium dialysis to find a binding constant 
for a product to an enzyme.  I only plan this to be a one time thing and 
was wondering if there was a reference out there that explains the set up 
and maybe how to do this simply with things in the lab instead of buying 
an equilibrium dialysis setup.

I expect the Kd to be in the submicromolar range and the enzyme is soluble.

Any help appreciated.  Thanks in advance.

Bill