Equilibrium dialysis

William P. Tschantz tschantz at galactose.mc.duke.edu
Tue Mar 5 16:33:48 EST 1996


I have a question about equilibrium dialysis to find a binding constant 
for a product to an enzyme.  I only plan this to be a one time thing and 
was wondering if there was a reference out there that explains the set up 
and maybe how to do this simply with things in the lab instead of buying 
an equilibrium dialysis setup.

I expect the Kd to be in the submicromolar range and the enzyme is soluble.

Any help appreciated.  Thanks in advance.


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